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Question

Are sticky ends created by Hindlll?


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Solution

Hindlll

  1. Hindlll is a restriction enzyme.
  2. A restriction enzyme cleaves DNA into fragments at or near restriction sites within molecules.
  3. When certain restriction enzymes digest the DNA, the ends of a DNA strand are left with one strand overhanging the other, forming a short single-stranded segment.
  4. This overhang is known as "sticky ends" because it easily re-attaches to other similar ends.
  5. The restriction enzyme HindIII produces sticky ends.
  6. The recognition site is a palindrome with the letters AAGCTT in the sequence.
  7. It cuts the DNA between the two AA.

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