How do you get primers for PCR?

Open in App

Solution

Polymerase chain reaction:
It is a technique used in laboratories for the rapid production of a specific segment of DNA to make billions of its copies.
Polymerase chain reaction involves amplification of DNA sample using three basic steps.
The first step involves the denaturation of the DNA template to form single strands.
Synthesis of new strands by annealing the primers to each original strand is the second step.
The primers that are used for annealing are oligonucleotides that are synthesized artificially.
The last step involved in PCR is the extension of the newly formed DNA strands from the primers.

Suggest Corrections

Similar questions

Q. Following are the two statements regarding PCR primers.

Statement1: PCR primers must not have self-complementary regions.
Statement 2: Self-complementary regions result in hairpin structures, adversely affecting the PCR.

Q. Which of the following is favoured for designing primers that are required during PCR?

Q. (a) Mention the number of primers required in each cycle of polymerase chain
reaction (PCR). Write the role of primers and DNA polymerase in PCR., (b) Give the characteristic feature and source organism of the DNA polymerase in PCR.

What is primers? Mention its role and the number of primers required in each cycle of PCR. Give the characteristic feature and source organism of the DNA polymerase used in PCR.

Q. Assertion :PCR primers must not have self complementary regions. Reason: Self complementary regions result in hairpin structures adversely affecting the PCR.

Join BYJU'S Learning Program