Question

How to culture Rhizobium bacteria?

Answer 1

Rhizobium bacteria:

1. A bacteria called Rhizobium leguminosarum coexists mutualistically and symbiotically with legumes.
2. It can transform air nitrogen into a form that plants can use.
3. Rhizobium leguminosarum bv. a soil bacterium.

Steps to culture Rhizobium bacteria:

1. Carefully remove any legume plant to get rid of muck or soil, and rinse its roots with sterile water.
2. Use a clean knife to trim the nodules that you can see on the roots.
3. Put it in a jar with pristine water.
4. After washing the nodules, place them for a few minutes in a jar with mercuric chloride solution (removes microbes if any on the nodule and disinfects it), nodules need to be repeatedly cleaned with alcohol and then with clean water.
5. Keep the nodule in a test tube right now. Drops of distilled water should be added.
6. To discharge bacteria into the water, break up nodules using a glass rod.