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Question

What are the parameters which are characteristics for DNA extraction procedures?


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Solution

DNA extraction procedures:

  1. DNA extraction is a technique that uses physical and/or chemical processes to separate DNA from cell membranes, proteins, and other biological components in a sample.
  2. The three parameters that are commonly used in DNA extraction procedures are as follows:
    1. The length of target sequences for studies is typically between 100 and 400 bp. DNA quantity refers to the total amount of extracted DNA.
    2. Elevated DNA purity is needed to avoid complications during subsequent analyses.
    3. After extracting the DNA, it can be used in molecular studies such as PCR, electrophoresis, sequencing, fingerprinting, and cloning.

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