What Are Northern Blots Based On?
Northern blots are based on nucleic acid hybridization, the difference being that RNA is the target in the northern blot.... View Article
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What Are Southern Bolts Used For?
It is used to find how closely related DNA is from one source compared to a sequence of DNA from... View Article
What Does The Hybridization Of DNA Or RNA Entail?
If two differing double helixes of DNA melt, before reannealing and cooling, the single strands can be mixed together. If... View Article
What Is The Role Of Biotin In Labelling DNA?
Biotin is a vitamin, digoxigenin is a steroid from the plant of foxglove. With the help of these chemicals, scientists... View Article
How To Detect Radioactive DNA?
They can be identified with the help of scintillation counting where small samples of the radioactive DNA are blended with... View Article
Give One Means Of Requiring The Ability To Detect DNA.
The recombinant DNA methodologies necessitate the capability to detect DNA. One of the ways to find the amount of RNA... View Article
What Happens When 2 Different DNA Samples Are Snipped With A Similar Sticky-end Restriction Enzyme?
All the fragments tend to have identical overhangs when two varying samples of DNA are snipped with a similar sticky-end... View Article
What Is Considered To Be Key To Make DNA Fragments?
The key to making DNA fragments is restriction enzymes that naturally occur or the restriction endonucleases. Such bacterial enzymes associate... View Article
What Is Pulsed-field Gel Electrophoresis?
The type of gel used is affected by the size of the DNA which is examined. The standard one is... View Article
Brief About The Separation Of Molecules In Cloning.
Once sequencing reactions take place, the mixture of strands of varying lengths and all endings of ddNTP (fluorescently labelled) has... View Article
When Is A Gene Said To Be Cloned?
Producing exact genetic copies of an entire entity, fragments of DNA or cells are referred to as clones. Clones are... View Article
What Is The Principle Of PCR And Sequencing?
Principle of PCR – The objective of Polymerase chain reaction (PCR) is to produce a great number of copies of... View Article
Brief About The Method Involved In Gene Cloning.
Method – Desired DNA or gene, with the help of restriction enzymes, are isolated Both plasmid and desired gene are... View Article
Explain The Insertion And Plasmid Multiplication Step Of Genetic Engineering?
Insertion of the plasmid into the cell again – in this step, the circular plasmid DNA which now comprises the... View Article
What Does The Step Isolation Of Gene And Preparation In Genetic Engineering Include?
Genetic engineering includes several steps, two of which are – Isolation of genes – genes to produce a protein is... View Article
How To Identify A PCR Product?
A variation in one of the PCR techniques can be used in the determination of evolutionary relationships between organisms. The... View Article
What Is Quantitative PCR-Q-PCR?
It is a method used in the quick assessment of the measure of PCR product (real-time preferably). As a result,... View Article
What Is A Reverse Transcription PCR?
Reverse Transcription PCR (RT-PCR) is a procedure made for identification, isolation and amplification of a known sequence forming a cell... View Article
What Are Touchdown PCR And Colony PCR?
Touchdown PCR is a variant of PCR that decreases the nonspecific primer annealing. This is done by lowering the annealing... View Article
What Is A Methylation-specific PCR?
It is used in the detection of methylation of the CpG islands in the genomic DNA. Here, DNA is treated... View Article
