Effect of Temperature on Enzymes

Q.

What are the Four Enzyme Characteristics?

Q.

Are enzymes destroyed by high heat?

Q.

Assertion (A) All enzymes are proteins but all proteins are not enzymes.

Reason (R) Enzymes are biocatalysts and have a stable configuration having an active site.

1. Both A and R are correct but R is the correct explanation for A

2. Both A and R are correct but R is not the correct explanation for A

3. A is correct but R is not correct

4. A is incorrect but R is correct.

Q. What happens to any chemical reaction when the temperature is increased?

1. Its rate increases
2. Its rate decreases
3. It goes at a slower rate
4. It stops

Q.

Enzymes are proteins. Proteins are long chains of amino acids linked to each other by peptide bonds. Amino acids have many functional groups in their structure.

These functional groups are many of them at least, ionisable. As they are weak acids and bases in chemical nature, this iohisation is influenced by pH of the solution. For many enzymes, activity is influenced by surrounding pH. This is depicted in the curve below, explain briefly.

Q.

What are the types of specificity?

Q.

How does the hydrogen ion concentration affect enzyme activity?

Q.

What is the smallest enzyme in the human body?

Q. What are enzymes? Enumerate the properties of enzymes.

Q.

The temperature at which there is a maximum activity of the enzymes in humans is ${37}^{\circ }C$. The most plausible reason for this is

1. The activity is maximum at 37 degrees for all enzymes

2. The optimum temperature varies for every enzyme.

3. Higher temperatures can denature the proteins

4. It is the physiological temperature and is optimum for the activity of enzymes in the human body

Q.

What are enzyme characteristics?

Q. Explain the factors affecting enzyme activity.

Q.

Enzymes with two sites are called

1. Apoenzyme.

2. Holoenzyme

3. Conjugate enzyme

4. Allosteric enzyme

Q.

What are the 3 features of an enzyme?

Q.

Regarding the graph, assertion and reason; choose the correct option, where X Axis is Temperature and Y axis is Enzyme activity.

Assertion [A]: The graph of Rate of Enzyme activity vs Temperature is always a
parabola.

Reason [R]: Enzyme activity always increases with rise in temperature.

\( x \)-axis

Q. For animal enzyme to act properly, the following temperature is required

1. 
2. 
3. 
4. .

Q. Observe the graph and answer the following questions.
The curve given below show enzymatic activity with relation to three
conditions (pH, temperature and substrate concentration), what do the axes (x and y) represent?

1. x-axis = Enzymatic activity, y-axis = Temperature
2. x-axis = Enzymatic activity, y-axis = pH
3. x-axis = Substrate concentration, y-axis = Enzymatic activity
4. x-axis = Temperature, y-axis = Enzyme activity

Q. What happens to any chemical reaction when the temperature is increased?

1. Its rate decreases
2. Its rate increases
3. It stops
4. It goes at a slower rate

Q. Assertion :Low temperature destroys enzymes by causing their denaturation. Reason: High temperature preserves the enzymes in their inactive stage.

1. Both statements 1 and 2 are correct
2. Statement 1 is correct but statement 2 is incorrect
3. Statement 1 is incorrect but statement 2 is correct
4. Both statements 1 and 2 are incorrect.

Q. Select the correct match of enzymes listed, their site of action, their substrate and optimum $pH$

Enzyme	Site of action	Substrate	pH
Amylopsin	Pancreas	Starch	$7.8$
Trypsinogen	Duodenum	Peptones	$6.8$
Nucleotidase	Small intestine	Nucleoside	$6.8$
Carboxypeptidase	Small intstine	Polypeptide	$7.8$

Q. Temperature is increased from $3^o$ to ${40}^{o}C$. The rate of enzyme controlled biochemical reaction will

1. Not change
2. Increase
3. Increase initially and then decrease
4. Decrease

Q.

The fastest enzyme is

1. Urease

2. Carbonic anhydrase

3. Trypsin

4. Pepsin

Q. Sir a crazy looking doubt
sir the temperatures around the earth are gradually increasing and after a certain point...we may be continuously sweating for days together(may happen).so humans may evolve in the coming thousand years like increasing the body temperature according to surrounding.then there may be a decrease in enzymatic activity so enzymes may change where they'll word at their highest level at then maintained body heat, etc..
Is this idea fundemently correct?

Q.

The structure of a protein can be denatured by:

1. Heat

2. The presence of oxygen

3. The polar bonds of water molecule

4. The presence of carbon dioxide

Q. Three of the following statements about enzymes are correct and one is wrong. Which one is wrong.

1. Enzymes require optimum pH for maximal activity
2. Enzymes are denatured at high temperature but in certain exceptional organisms they are effective even at ${80}^o-{90}^{o}C$
3. Most enzymes are proteins but some are lipids
4. Enzymes are highly specific

Q.

The temperature at which there is a maximum activity of the enzymes in humans is ${37}^{\circ }C$. The most plausible reason for this is

1. It is the physiological temperature and is optimum for the activity of enzymes in the human body

2. Higher temperatures can denature the proteins

3. The activity is maximum at 37 degrees for all enzymes

4. The optimum temperature varies for every enzyme.

Q. Optimum temperature is the temperature at which an enzyme

1. Works at its best
2. Is not destroyed
3. Action is reversed
4. Is inactivated

Q. Optimum temperature for the enzyme activity for most enzymes is

1. ${20}^o-{35}^{o}C$
2. ${15}^o-{25}^{o}C$
3. ${40}^o-{65}^{o}C$
4. ${30}^o-{45}^{o}C$

Q. The curve given here shows enzymatic activity with relation to three conditions (pH, temperature and substrate concentration). What do the two axes, $X$ and $Y$ represent?

1. $X$-axis - Substrate concentration, $Y$-axis - Enzyme activity
2. $X$-axis - Enzyme activity, $Y$-axis - Temperature
3. $X$-axis - Enzyme activity, $Y$-axis - pH
4. $X$-axis - Temperature, $Y$-axis - Enzyme activity

Q. Which of the following factor does not affect an enzyme's activity?

1. pH levels
2. Inhibitors
3. Temperature
4. Enzyme cofactors
5. Enzyme concentration