Gram Staining

Q. Gram' staining is a common technique used to differentiate two large groups of bacteria based on their different

1. Cell membrane
2. Extracellular matrix
3. Cell wall
4. All of the above

Q. While performing the Gram staining, what type of specimen is used?

1. Live
2. Heat-fixed
3. Unstained
4. Those sectioned into thin slices

Q. The dyes that are used in Gram's staining are

1. Crystal violet and methylene blue
2. Crystal violet and safranin
3. Safranin and methylene blue
4. Crystal violet and carbol fuchsin

Q. Gram staining technique is based on the fact that Gram-positive bacteria retain _______ stain that Gram-negative bacteria do not.

1. Safranin
2. Crystal violet
3. Acetone
4. Phenol

Q. In Gram staining technique, Gram-positive bacteria

1. appear violet even after the addition of counterstain
2. do not get stained by crystal violet
3. take up the counterstain and appear orange-red
4. lose crystal violet stain on washing with alcohol.

Q. In Gram staining technique, Gram-positive bacteria

1. do not get stained by crystal violet
2. appear violet even after the addition of counterstain
3. take up the counterstain and appear orange-red
4. lose crystal violet stain on washing with alcohol.

Q. In Gram staining technique, Gram-positive bacteria

1. do not get stained by crystal violet
2. appear violet even after the addition of counterstain
3. take up the counterstain and appear orange-red
4. lose crystal violet stain on washing with alcohol.

Q. Teichoic acid is present in

1. Gram +ve bacteria
2. Gram -ve bacteria
3. Spirochaete
4. Actinomycetes

Q. What are Gram-Positive bacteria?

Q. During Gram's stain

1. All bacteria whether Gram (+)ve or (-)ve, take crystal violet stain
2. Only Gram +ve bacteria take crystal violet stain
3. Only Gram (-)ve bacteria take crystal violet stain
4. Gram (+)ve bacteria lose this stain after alcohol treatment and take red stain of safranin