Limitation of Plasmid Vector

Q. Which of the following are limitations of using plasmids as vectors?

1. Inability to uptake large insert size
2. Inability to express eukaryotic genes efficiently
3. Inability to express prokaryotic genes
4. Ability to uptake small insert size

Q. What is the biggest disadvantage of plasmids?

1. They work only in bacteria and fungi
2. Restriction enzymes can cut it
3. DNA segments bigger than 7 Kbp cannot be inserted
4. Their functioning is not yet fully studied

Q. What is the biggest disadvantage of plasmids?

1. They work only in bacteria and fungi
2. Restriction enzymes can cut it
3. DNA segments bigger than 7 Kbp cannot be inserted
4. Their functioning is not yet fully studied

Q. Select the option that excludes characteristics applicable to plasmids
(a) Circular DNA
(b) Linear DNA
(c) Present in all bacteria
(d) Contain essential genes\
(e) Extra chromosomal self-replicating

1. b and d only
2. b, c and d only
3. d, b, a and c
4. a only

Q. Which of the following contain plasmids, which can be used to insert DNA fragments into cells, for transgenic animals production?

1. Viruses
2. Bacteria
3. Fungi
4. Plant cells
5. Animal cells

Q. Which one of the following statements is not correct about a plasmid?

1. It is a circular DNA.
2. It has the ability of autonomous replication.
3. Its DNA is as long as chromosonal DNA.
4. It has antibiotic resistant gene.

Q. Plasmids are preferred as vehicle DNA because they

1. denature easily
2. can be easily detected at time of cloning experiments
3. multiply along with bacterial chromosome
4. can be easily isolated and introduced

Q. Select the option that excludes characteristics applicable to plasmids.
a. Circular DNA
b. Linear DNA
c. Present in all bacteria
d. Contain essential genes
e. Extra chromosomal self-replicating

1. d, b, e and c only
2. a only
3. b, c and d only
4. b and d only

Q. The small, circular extrachromosomal DNA called plasmid is found in

1. Viruses
2. Bacteria
3. Fungi
4. Plant cells
5. Animal cells

Q. What is the feature of plasmid used as cloning vector?

Q. Which of the following are limitations of using plasmids as vectors?

1. Inability to uptake large insert size
2. Inability to express eukaryotic genes efficiently
3. Inability to express prokaryotic genes
4. Ability to uptake small insert size

Q. Which of the following statement is true regarding plasmids?

1. Plasmids are widely used in gene transfer.
2. Plasmid contains gene for vital activities.
3. These are main part of chromosome.
4. These are found in virus.

Q. The presence of an extrachromosomal circular DNA called as a plasmid is a characteristic feature of

1. prokaryotes
2. plants
3. animals
4. fungi

Q. Which of the following are limitations of using plasmids as vectors?

1. Inability to uptake large insert size
2. Inability to express eukaryotic genes efficiently
3. Inability to express prokaryotic genes
4. Ability to uptake small insert size

Q. What is true of plasmid?

1. Found in viruses
2. Contains genes for vital activities
3. Part of nuclear chromosome
4. Widely used in gene transfer

Q. The genes which on activation produce malignant neoplasm are

1. Pleiotropic genes
2. Oncogenes
3. Neonatal genes
4. Multiple genes

Q. Maximum number of bases in plasmids is

1. $50$ kilobase
2. $5000$ kilobase
3. $500$ kilobase
4. $50,000$ kilobase

Q. Which of the following statement is false?

1. $T_4$ bacteriophage has double stranded DNA molecule.
2. Most animal viruses are DNA viruses.
3. Most plant viruses are RNA viruses.
4. TMV has a doubled stranded RNA.

Q. What is the biggest disadvantage of plasmids?

1. They work only in bacteria and fungi
2. DNA segments bigger than 7 Kbp cannot be inserted
3. Their functioning is not yet fully studied
4. Restriction enzymes can cut it

Q. RNA interference which is employed in making tobacco plant resistant to Meloidegyne incognita is essentially involved in

1. preventing the process of replication of DNA
2. preventing the process of translation of mRNA
3. preventing the process of splicing of hnRNA
4. preventing the process of transcription