Ori and Replication Fork

Q. The specific DNA sequence in the genome of an organism at which DNA replication gets initiated is called

1. Ori
2. RNA primer
3. origin of replication
4. Okazaki fragment

Q. Replication fork is

1. The site at which DNA replication is initiated
2. The junction between the newly separated DNA template strands and the unreplicated DNA duplex
3. The Okazaki fragment together with the respective template strand
4. Synonymous to ori site

Q. In prokaryotic cells, there is only one point of origin of replication and eukaryotic cells have multiple points of origin.

1. True
2. False

Q.

Describe briefly the following

(a) Origin of replication

(b) Bioreactors

(c) Downstream processing

Q. A DNA molecule in which both strand have radioactive thymidine is allowed to duplicate in an environment containing non-radioactive thymidine. What will be the correct number of DNA molecules that contain some radioactive thymidine after three duplications?

1. There will be two such molecules.
2. There will be eight such molecules.
3. There will be only one such molecule.
4. There will be four such molecules.

Q.

The one which is capable of self replication is

1. DNA
2. RNA
3. Enzyme
4. Protein

Q. Animal cells uses thymine primarily for the synthesis of DNA. A group of sea urchin eggs was fertilized in sea water containing radioactive thymine. Following fertilization, samples of embryos were removed at regular intervals and the radioactivity in the nucleic acid was measured in counts per minute. The results obtained are shown in the given figure.
The radioactivity of the embryos increases with time probably results from

1. Synthesis of new proteins by the developing embryos
2. Synthesis of radioactive thymine by the developing embryos
3. Oxidation of radioactive thymine
4. Incorporation of radioactive thymine in new cell membranes
5. Incorporation of radioactive thymine in new DNA during replication

Q. Given below is the transcribed strand of the DNA duplex :3'-TACCGATCCGAGCTG-5'
(a)Draw the complementary DNA polynucleotide chain
(b)Construct the RNA molecule, which will'be transcribed.

Q. DNA multiplication is also called as

1. Replication
2. Transduction
3. Transcription
4. Translation

Q. In bacterial DNA replication, synthesis starting from the site of origin of replication

1. Involves RNA primers
2. Requires telomerase
3. Proceeds unidirectionally
4. Moves bidirectionally