Process of Transcription

Q. Which of the following is TRUE?

1. DNA polymerase reads in 5’-3’ direction and RNA polymerase reads in 5’-3’ direction
2. DNA polymerase reads in 5’-3’ direction but RNA polymerase reads in 3’-5’ direction
3. DNA polymerase reads in 3’-5’ direction and RNA polymerase reads in 3’-5’ direction
4. DNA polymerase reads in 3’-5’ direction, but RNA polymerase reads in 5’-3’ direction

Q. The term refers to the sequence of DNA that is copied during the synthesis of mRNA.

1. leading strand
2. template strand
3. coding strand
4. lagging strand

Q.

Look at the following complementary sequences of a single DNA molecule, and identify the correct mRNA sequence that gets transcribed from it:

5’ TAGGCGCATT 3’

3’ ATCCGCGTAA 5’

1. 5’ UAGGCGCAUU 3’

2. 3’ AUCCGCGUAA 5’

3. 3’ UAGGCGCAUU 5’

4. 5’ TAGGCGCATT 3’

Q. Sort the events that occur during transcription

1. Termination
2. Elongation
3. Capping
4. Initiation
5. Poly A tailing

Q. mRNA is synthesised on DNA template in which direction:

1. $5^{\prime }\to 3^{\prime }$
2. $3^{\prime }\to 5^{\prime }$
3. Both
4. Any

Q. Choose the wrong statement in the process of protein synthesis.

1. Translation is the process, in which proteins are synthesised from the RNA
2. In the presence of DNA polymerase enzyme, the mRNA is formed based on the triplet codes
3. After uncoiling of DNA molecule, one strand acts as a template for the formation of mRNA
4. The mRNA that leaves nucleus reached cytoplasm and gets attached with 30S ribosomal subunit
5. The amino acids are transferred from the intracellular amino acid pool to the active ribosomes by the tRNA

Q. Select the correct option:

1. Direction of RNA synthesis - 3' - 5' ; Direction of reading of the template DNA strand - 5' - 3'
2. Direction of RNA synthesis - 5' - 3' ; Direction of reading of the template DNA strand - 5' - 3'
3. Direction of RNA synthesis - 5' - 3' ; Direction of reading of the template DNA strand - 3' - 5'
4. Direction of RNA synthesis - 3' - 5' ; Direction of reading of the template DNA strand - 3' - 5'

Q. During transcription, RNA polymerase holoenzyme binds to a gene promoter and assumes a saddle-like structure. What is its DNA-binding sequence?

1. TATA
2. TTAA
3. AATT
4. CACC

Q.

Briefly describe the following:
(a) Transcription

(b) Polymorphism

(c) Translation

(d) Bioinformatics

Q.

If the sequence of the coding strand in a transcription unit is written as follows
5' - ATGCATGCATGCATGCATGCATGCATGC - 3'
Write down the sequence of mRNA.