Promoter Sequence

Q.

Differentiate between the followings:

(a) Repetitive DNA and Satellite DNA

(b) mRNA and tRNA

(c) Template strand and Coding strand

Q. The region of DNA sequence that provides binding site for RNA polymerase is

1. Structural gene
2. Origin sequence
3. Terminator
4. Promoter

Q.

Explain (in one or two lines) the function of the followings:
(a) promoter

(b) tRNA

(c) Exons

Q. (a) Absence of lactose in the culture medium affects the expression of a lac-operon in E.coli. Why and how? Explain.

(b) Write any two ways in which the gene expression is regulated in eukaryotes. [5]

Q. The distinction of “upstream” in a DNA strand lies between

1. Promoter and Terminator
2. The last Exon and Terminator
3. Promoter and Transcription unit
4. The first Intron and the next Exon

Q. In double helix of DNA, the two DNA strands are

1. Coiled around a common axis
2. Coiled over protein sheath.
3. Coiled around each other
4. Coiled differently

Q. Bacteria protect themselves from viruses by fragmenting viral DNA with

1. Gyrase
2. Ligase
3. Endonuclease
4. Exonuclease

Q.

What is meant by tailing?

Q. Preetha was practising PCR hands-on in her laboratory. She successfully isolated the gene of interest (GOI), however the quantity was too low. She desired to amplify the sequence. She conducted a PCR after which she tried to check for amplification through gel electrophoresis. She observed that all the lanes of the gel contained two bands, A and B.


Given that B was comparatively closer to the positive terminal, predict the correct alternative.

1. A band is of GOI, B band is of primer-dimer
2. B band is of GOI, A band is of primer-dimer
3. Both A and B are primer-dimers
4. Both A and B are bands of GOI

Q. Topoisomerase is involved in

1. Producing RNA primer
2. Producing nick in DNA
3. Joining of DNA segments
4. Separation of DNA strands.

Q.

Differentiate between the process of transcription in prokaryotes and eukaryotes ?

Q. Match column-I with column-II and choose the correct option.

1. 1-(A), 2-(B), 3(C)
2. 1-(C), 2-(A), 3-(B)
3. 1-(B), 2-(C), 3-(A)
4. 1-(C), 2-(B), 3-(A)

Q.

distinguish between RNA polymerase and DNA polymerase

Q. The TATA box is also known as the _________ region.

1. Terminator
2. Promoter
3. Repressor
4. Transcription unit

Q.

____(A)______ contains a single ______(B)________ and several ____(C)____ attached to it.

1. A- Polysome, B - mRNA, C -ribosomes

2. A - Polysome, B - ribosome, C -mRNAs

3. A - Transcription unit, B - mRNA, C - enzymes
4. A - Transcription unit, B - enzyme, C - mRNAs

Q. Following is an image that depicts PCR cycle and its important components. Identify i, ii, iii, and iv in the same.

1. i - Primers, ii - Deoxyribonucleotides, iii - 96℃, iv - Annealing
2. i - Deoxyribonucleotides, ii - Primers, iii - 96℃, iv - Annealing
3. i - Primers, ii - Deoxyribonucleotides, iii - 72℃, iv - Annealing
4. i - Primers, ii - Taq polymerase, iii - 96℃, iv - Annealing

Q. Differentiate between :
Promoter and terminator in a transcription unit.

Q. Which of the following is not a function of the repressor?

1. Blocks passage of RNA polymerase towards structural enzyme.
2. Has two allosteric sites.
3. Changes the shape of the operator binding site.
4. None of these.

Q.

Explain (in one or two lines) the function of the followings:

(a) Promoter

(b) tRNA

(c) Exons

Q. During transcription, holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called ?

1. AAAT box
2. TATA box
3. GGTT box
4. CART box

Q.

Which end of the template DNA is the TATA sequence present on?

1. 3’ downstream

2. 5’ downstream

3. 3’ upstream

4. 5’ upstream

Q. Make a list of plasmids which make sticky ends and which make blunt ends?

Q. Mention the function of promoter gene in transcription.

Q. Which of the following describes the process seen in the image above?

1. The TATA box is used as a signal for the termination of transcription.
2. The enhancer region is upstream from the binding site of RNA polymerase which allows transcription factors to bind and initiate transcription.
3. The enhancer region acts to loop up DNA and therefore, silence gene expression.
4. The TATA region of the DNA is known as the promoter, which signals DNA polymerase to start transcribing.

Q. What are the differences between ends produced in dna strands in blunt ends and by restriction nucleases?

Q. How many adenines does the TATA box consist of, in total?

1. 3
2. 6
3. 4
4. 5

Q. How many adenines does the TATA box consist of, in total?

1. 3
2. 4
3. 5
4. 6

Q. Starting codon is universal which means

1. It is the universally same for all organisms
2. Two or more codons
3. Any variable code is called starting codon which can start protein synthesis
4. All codons originated from same starting codon

Q. How many adenines does the TATA box consist of, in total?

1. 3
2. 4
3. 5
4. 6

Q. The enzyme responsible for transposition is

1. Transposase
2. Ligase
3. Recombinase
4. Endonuclease