Question

A cell is made competent to take up foreign DNA using the following methods except ?

• A. A microinjection.
• B. Biolistics
• C. Bivalent $C{a}^{++}$
• D. Monovalent $N{a}^{+}$

Answer 1

Answer: (D)

Monovalent $N{a}^{+}$

A cell is made competent or enabled to take up a foreign for obtaining recombinant DNA using a microinjection (a glass pipette for transferring foreign DNA into the target cell. Biolistic method uses a gene gun to transfer DNA coated gold particle into the cell. Similarly use of divalent ion $C{a}^{++}$ also assists with uptake of foreign DNA by the cell.

So, the correct answer is 'Monovalent

Na+