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Question

A mixture containing DNA fragments, A, B, C and D, with molecular weights of A + B = C, A > B and D > C, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anode sides of the gel would be

A
D, C, A, B
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B
A, B, C, D
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C
C, B, A, D
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D
B, A, D, C.
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Solution

The correct option is C D, C, A, B
  • Shorter lengths of DNA move faster than longer lengths so move further in the time the current is run. So, since D has the heaviest weight (implying it has the longest strand), it will tend to remain far from the anode. B being a lightest fragment moves farthest from the cathode.
  • Hence The positions of these fragments from cathode to anode sides of the gel would be D, C, A, B,
  • So,the correct answer is 'D,C,A,B'.

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