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Question

A mixture DNA fragments A, B, C and D, with molecular weights of A+B=C>B and D>C was subjected to agarose get electrophoresis. The position of these fragments from cathode to anode sides of the gel would be.

A
B, A, C, D
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B
A, B, C, D
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C
C, B, A, D
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D
B, A, D, C
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Solution

The correct option is A B, A, C, D
The positions of these DNA fragments on the electrophoresis gel depends upon relative molecular weight.
The fragment with the lowest molecular weight will appear farther from the wall and fragment with higher molecular weight will appear near the wall. So, on the basis of information given the molecular weight of d is maximum and the molecular weight of b is minimum. So, the order of fragment will be (b, a, c, d).
So, the correct option is 'A'.

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