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Question

A scientist is trying to engineer a plasmid for purposes of genetically modifying crops.
What is the most appropriate first step in the process?

A
Identify the gene of interest that will confer a benefit in the genetically modified crop.
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B
Cut both the plasmid and the gene with the same restriction enzyme.
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C
Use ligase to anneal the gene in the bacterial chromosome.
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D
Select the bacteriophage that will be used as a vector for gene delivery.
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Solution

The correct option is A Identify the gene of interest that will confer a benefit in the genetically modified crop.
Plasmid can be used as a vector for transferring a foreign gene of desired property in to a plant, the gene is required to be identified and amplified by PCR or other similar technique and then inserted into the plant.
So, the correct answer is 'dentify the gene of interest that will confer a benefit in the genetically modified crop'

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