Question
A scientist isolated his gene of interest and found it to be 660 kb in size. Which of the following vectors should he use to transfer the gene of interest?
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Solution
The correct option is C YAC
The insert size in plasmid vectors cannot exceed 6 - 7 kb. Cosmids can be used to clone DNA inserts up to 45kb. Other vectors like the Bacterial artificial chromosome (BAC) can accommodate up to 300-350kb of foreign DNA and Yeast artificial chromosome (YAC) can clone DNA fragments of more than 1Mb in size. Therefore, in this case, the scientist should use YAC as a vector.
The insert size in plasmid vectors cannot exceed 6 - 7 kb. Cosmids can be used to clone DNA inserts up to 45kb. Other vectors like the Bacterial artificial chromosome (BAC) can accommodate up to 300-350kb of foreign DNA and Yeast artificial chromosome (YAC) can clone DNA fragments of more than 1Mb in size. Therefore, in this case, the scientist should use YAC as a vector.
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