The correct option is A Formation of fertilization membrane
The vitelline membrane is the membrane, which is adjacent to the outer surface of the plasma membrane of an ovum. When the spermatozoa fuses with the ovum, there is signal transduction, which results in deposition of several substances onto vitelline membrane and this get transformed into hard layer, which is called as the fertilization membrane.
Thus, the correct answer is option D, 'Formation of fertilization membrane'.