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Question

An analysis of chromosomal DNA using the Southern hybridization technique does not use:
  1. Electrophoresis
  2. Blotting
  3. Autoradiography
  4. PCR


Solution

The correct option is D PCR
An analysis of chromosomal DNA using the Southern hybridization technique involves the three following steps:
  • Gel electrophoresis
  • Blotting
  • Autoradiograph
PCR or polymerase chain reaction is used to amplify a single copy or a few copies DNA of interest. This process is not employed during a Southern hybridization technique.

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