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Question

An inhibitor is added to a cell culture so that succinate accumulates. The enzyme catalysing the formation of which substance has been blocked?

A
Citrate
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B
Oxaloacetate
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C
α-ketoglutarate
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D
Fumarate
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Solution

The correct option is A Citrate
Succinate was placed in one of the most important cyclic pathways in metabolism, a collecting pool of catabolic and a starting point of many anabolic processes. Succinate is a product of substrate-level phosphorylation materialized in the citric acid cycle. It is involved in a macrophage-specific metabolic pathway generating and is also a downstream product of the α-ketoglutarate dehydrogenase complex, a heavily regulated multi-subunit complex.
So the correct answer is 'Citrate'.

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