CameraIcon
CameraIcon
SearchIcon
MyQuestionIcon


Question

Arrange the following DNA samples in ascending order of their melting temperature (Tm)?
i. DNA with 13% T
ii. DNA with 26% T
iii. DNA with 30% T
iv. DNA with 20% T


Your Answer
A
i, ii, iv, iii
Correct Answer
B
iii, ii, iv, i
Your Answer
C
i, ii, iii, iv
Your Answer
D
i, iv, ii, iii

Solution

The correct option is D iii, ii, iv, i
Tm is the temperature at which half of the DNA duplex is denatured. Tm is directly proportional to G-C content.
Tm is less for DNA with high A/T content because A and T are bound by a double hydrogen bond while G and C are bound by a triple hydrogen bond which makes it stronger and difficult to break..
So, DNA with 30% T would have lowest Tm.

flag
 Suggest corrections
thumbs-up
 
0 Upvotes


Similar questions
View More


People also searched for
View More



footer-image