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Question

Arrange the order of events of initiation of translation. I. AUG or GUG comes to lie over P-site to produce 40S-mRNA complex.
II. P-site attracts tRNA which lies opposite to initiation codon to form 40S-mRNA-tRNAmet.
III.Union of the two subunits of ribosomes occurs, A site becomes operational and second codon of mRNA lies over it.
IV. mRNA attaches itself to smaller subunit of ribosome with its cap coming in contact with 3’ end of 18 S rRNA.
V. The larger subunit of ribosome now attaches to 40S-mRNA-tRNAmetcomplex to form 80S mRNA-tRNA complex.
a. I, II, III, IV, V
b.II, V, III, IV, I
c. III, IV, V, I, II
d. IV,I,II,V,III
  1. a
  2. b
  3. c
  4. d


Solution

The correct option is D d

mRNA attaches itself to smaller subunit of ribosome with its cap coming in contact with 3’ end of 18 S rRNA. AUG or GUG comes to lie over P-site to produce 40S-mRNA complex.P-site now attracts met tRNA. The anticodon of tRNA comes to lie opposite to initiation codon. It gives rise to form 40S-mRNA-tRNAmet.The larger subunit of ribosome now attaches to 40S-mRNA-tRNAmet complex to form 80S mRNA-tRNA complex. Mg2+ is essential for union of the two subunits of ribosomes. A site becomes operational. Second codon of mRNA lies over it.

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