Assertion :Complementary pairing between nucleotides is used to diagnose presence of a specific DNA segment in a mixture. Reason: DNA probes having radioactive isotopes help to detect DNA by autoradiography.

Both Assertion and Reason are correct and Reason is the correct explanation for Assertion

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Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion

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Assertion is correct but Reason is incorrect

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Both Assertion and Reason are incorrect

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Solution

The correct option is B Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
DNA probes are single DNA molecules of known sequences that have radioactive isotopes. They are used to detect the presence of a specific DNA segment complementary to their base sequences. Here, the complementary pairing between base nucleotides proves useful as the probe DNA and DNA to be detected being complementary to each other by hydrogen bonds between the base pairs and form a double-stranded DNA molecule. This double standard DNA can be easily detected by autobiography due to the radioactivity of isotopes present in the DNA probe.

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