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Question
Assertion: Pore size in the matrix used for gel electrophoresis ranges between 50 and 200 nm.
Reason: Pore size of agarose gel depends on the concentration of agarose.
Assertion: Pore size in the matrix used for gel electrophoresis ranges between 50 and 200 nm.
Reason: Pore size of agarose gel depends on the concentration of agarose.Open in App
Solution
The correct option is A Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
Agarose molecules at high temperature have a random coil structure, but on cooling agarose chains form helical fiber bundles held together by non-covalent hydrogen bonds. As the temperature gets even lower, the fiber bundles get linked together by the formation of additional hydrogen bonds. The sizes of the pores, which typically range from 50 to 200 nm, depend on the concentration of agarose, size is inversely proportional to the concentration of agarose.
Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
Agarose molecules at high temperature have a random coil structure, but on cooling agarose chains form helical fiber bundles held together by non-covalent hydrogen bonds. As the temperature gets even lower, the fiber bundles get linked together by the formation of additional hydrogen bonds. The sizes of the pores, which typically range from 50 to 200 nm, depend on the concentration of agarose, size is inversely proportional to the concentration of agarose.
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