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Assertion :The direct ELISA differs from the indirect ELISA. Reason: Indirect ELISA involves the antigen binding to the primary antibody which then is detected by a labelled secondary antibody.
Assertion :The direct ELISA differs from the indirect ELISA. Reason: Indirect ELISA involves the antigen binding to the primary antibody which then is detected by a labelled secondary antibody.
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Solution
The correct option is A Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. ELISA can be performed by different methods, direct, indirect and sandwich. The direct method differs from the indirect method on the basis of the analyte which is used for the detection. In direct ELISA, the antibodies are detected. In the indirect ELISA, the antigen is coated on the ELISA plate which binds to the primary antibody, which later binds to the conjugated secondary antibody used for the detection. Thus, the correct answer is option A.
The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. ELISA can be performed by different methods, direct, indirect and sandwich. The direct method differs from the indirect method on the basis of the analyte which is used for the detection. In direct ELISA, the antibodies are detected. In the indirect ELISA, the antigen is coated on the ELISA plate which binds to the primary antibody, which later binds to the conjugated secondary antibody used for the detection.
Thus, the correct answer is option A.
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