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Process of DNA Fingerprinting

__ blotting m...

Question

__ blotting method is used in DNA fingerprinting.

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Solution

Edwin Southern developed the Southern blotting method to detect specific sequences in DNA samples. In this method, DNA is broken down into fragments, STRs are identified in them and amplified. They are then separated by running on gel electrophoresis.

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Q. __ blotting method is used in DNA fingerprinting.

Which blotting is used in DNA Fingerprinting?

Q. DNA fingerprinting method is very useful for

Q. Some of the steps of DNA fingerprinting are given below. Identify the correct sequence from the options given.

A

. Electrophoresis of DNA fragments

B

. Hybridisation with DNA probe

C

. Digestion of DNA by RENs

D

. Autoradiography

E

. Blotting of DNA fragments to nitrocellulose membrane

Q. Arrange in a sequence the given protocol for DNA fingerprinting:
(a) Denaturating DNA

(c) Extraction of DNA

(d) Fragmenting DNA

(e) Separation of DNA fragments by electrophoresis

(f) Hybridisation with the probe

(g) Exposure on film to make a DNA fingerprint

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