The correct option is A Midgut of insect by alkaline pH
Toxin is produced by Bt cotton plant in inactive state. This toxin does not kill the bacteria, but when an insect ingests it, this toxin gets activated by alkaline pH in midgut of insect. The activated toxin, then binds to the surface of epithelial cells of the midgut and create pores that causes swelling, lysis and finally result in the death of the insect.