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Question

Cells are made competent by a process that uses

A
calcium chloride and micro injection
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B
potassium chloride and heat shock
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C
calcium chloride and heat shock
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D
potassium chloride and microinjection
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Solution

The correct option is C calcium chloride and heat shock
DNA is a hydrophilic molecule, it cannot pass through cell membranes. In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42 ̊C (heat shock), and then putting them back on ice. This enables the bacteria to take up the recombinant DNA.

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