During dark reaction for fixation of carbon, the three carbon atoms of each molecule of 3-phosphoglyceric acid (PGA) are derived from:

RuBP only

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C O_{2}

only

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RuBP +

C O_{2}

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RuBP +

C O_{2}

+PEP

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Solution

The correct option is C RuBP + $C O_{2}$
During the carboxylation phase of the Calvin cycle, Ribulose -1,5-bisphosphate (RuBP) ( $=$ Ribulose diphosphate) acts as $C O_{2}$ acceptor and 6 molecules of RuBP react with 6 molecules of $C O_{2}$ and 6 molecules of water giving rise to 12 molecules of 3-phosphoglyceric acid.

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The reaction which is responsible for the primary fixation of CO₂ is catalysed by

C O_{2}

C_{4}

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