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Virus

Each restrict...

Question

Each restriction endonuclease recognises a specific palindromic nucleotide sequence in the DNA. Explain.

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Solution

The restriction endonuclease is found in bacteria, it is a part of the defence system of bacteria. It restricts replication of viral DNA within the bacterial cell by digesting viral DNA at specific points, these sequences are repetitive thus called as Palindromic sequence.

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Q. (a) Write the palindromic nucleotide for the following DNA segment:
5-G AA TTC-3
(b)Name the restriction endonuclease that recognises the sequence

(C)How are sticky-ends produced ? Mention their role.

Q. Read the given statements and select the correct option.
Statement 1 : Restriction endonuclease enzymes recognise a specific palindromic nucleotide sequence in the DNA.
Statement 2 : Restriction endonuclease enzymes are called as molecular scissors or biological scissors.

Q. (a) Explain the significance of 'palindromic nucleotide sequence' in the formation of recombinant DNA.

(b) Write the use of restriction endonuclease in the above process. [3]

Q. Assertion :Restriction endonuclease recognize short palindromic sequence and cut at specific sites. Reason: When a restriction endonuclease acts on palindrome, it cleaves both the strands of DNA molecule.

Q. (a) Why are restriction endonucleases so called ? (b) What is a palindromic nucleotide sequence ? How do restriction endonucleases act on palindromic sites to create sticky ends ?

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