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Process of DNA Fingerprinting

Electrophores...

Question

Electrophoresis and Southern blotting techniques are used in

A

DNA Fingerprinting

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B

Gene synthesis

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C

Gene cloning

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D

All of these

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Electrophoresis and Southern blotting techniques are used in

Q. The technique of DNA fingerprinting involves
(A) Digestion of DNA by restriction enzymes
(B) Isolation of DNA
(C) Hybridisation
(D) Blotting
(E) Separation by electrophoresis
(F) autoradiography

Choose the correct order of the steps

Q. Arrange in a sequence the given protocol for DNA fingerprinting:
(a) Denaturating DNA

(c) Extraction of DNA

(d) Fragmenting DNA

(e) Separation of DNA fragments by electrophoresis

(f) Hybridisation with the probe

(g) Exposure on film to make a DNA fingerprint

Q. Southern blotting technique uses .......... paper for embedding DNA strands.

Q. Some steps of DNA fingerprinting are given below. Identify the correct sequence from the options given.
1) Electrophoresis of DNA fragments
2) Hybridisation with DNA probe
3) Digestion of DNA by RENs
4) Autoradiography
5) Blotting of DNA fragments to nitrocellulose membrane

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