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Question

Enumerate the steps involved in testing a green leaf for the presence of starch.

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Solution

(a) Dip the leaf in boiling water for a minute to kill the cells.
(b) Boil the leaf in methylated spirit over a water bath till it becomes pale-white due to the removal of chlorophyll.
(c) Then place it again in hot water to remove excess spirit.
(d) Spread the leaf in a dish and pour iodine solution on it. The presence of starch will be indicated by a blue-black colour. A leaf without starch will show brown colouration.

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