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Explain the o...

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Explain the origin of replication in cloning vector.

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Q. Assertion :All expression vectors are cloning vectors and vice versa. Reason: Expression vectors have at least the regulatory sequences i.e., promoters, operator ribosomal binding sites, etc. having optimum function in the chosen control but not origin of replication.

Q. Which of the following features are required to facilitate cloning into a vector?
a. Origin of replication (ori)
b. Selectable marker
c. Cloning site

Q. Explain the features of cloning vectors.

Q. Explain in detail the role of restriction endonuclease in formation of recombinant DNA. Draw a labelled diagram of E.Coli cloning vector pBR 322.

Q. Mention the uses of cloning vector in biotechnology.

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