Question

Find the correct match.

	Column I		Column II
a.	Bacillus thuringiensis	1.	Production of human insulin
b.	Escherichia coli	2.	Production of Bt toxin
c.	Thermus aquaticus	3.	Biocontrol agent
d.	Trichoderma	4.	Used in PCR

• A. a-3, b-4, c-1, d-2
• B. a-4, b-3, c-2, d-1
• C. a-1, b-2, c-3, d-4
• D. a-2, b-1, c-4, d-3

Answer 1

The correct option is D a-2, b-1, c-4, d-3

Bacillus thuringiensis- Production of Bt toxin
Bacillus thuringiensis is a bacterium which produces a crystal protein, protoxin, also called Bt toxin. It is an insecticidal toxin which kills insects of class Lepidoptera, Coleoptera and Diptera.

Escherichia coli- Production of human insulin
Escherichia coli is the bacterium used to produce recombinant insulin by cloning genes of human A and B peptides of insulin separately. In the end, disulfide bonds are created in between both the peptides.

Thermus aquaticus- Used in PCR
Thermus aquaticus is an archaebacteria from which thermostable DNA polymerase (also called Taq polymerase) is isolated. This thermostable polymerase is used in PCR.
It extends the primers by adding dNTPs during PCR which is carried out at 72°C and only a thermostable DNA polymerase could carry out the reaction without getting denatured.

Trichoderma- Biocontrol agent
Trichoderma are free-living fungi found colonising the roots of plants. The fungus acts against any plant pathogen that may attack the plants, thus conferring disease resistance to the plants. It acts as a biocontrol agent.


Final answer:
(c) a-2, b-1, c-4, d-3