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Question
Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Bacterial cells receives naked DNA from solution by the process of
Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Bacterial cells receives naked DNA from solution by the process of
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Solution
The correct option is E Transformation
Transformation is a transfer of genetic material from one bacterial strain to other without establishing a physical contact; bacteria take up the DNA present in the surrounding. Transduction is a transfer of genetic material from one strain to other via viral particles. The virus infects bacteria and released viral particles carry part of the bacterial genetic material which is then transferred to next host bacterium. Translation is the process of synthesis of polypeptides by polymerization of amino acids on ribosomes using the mRNA triplets as a template. Electroporation is the method of direct gene transfer by applying a pulse of high voltage applied to protoplasts/cells/ tissues to make transient pores in the plasma membrane which serve to uptake the foreign DNA. Transpiration is the loss of water from stomata. Thus, the correct answer is option E.
Transformation is a transfer of genetic material from one bacterial strain to other without establishing a physical contact; bacteria take up the DNA present in the surrounding. Transduction is a transfer of genetic material from one strain to other via viral particles. The virus infects bacteria and released viral particles carry part of the bacterial genetic material which is then transferred to next host bacterium. Translation is the process of synthesis of polypeptides by polymerization of amino acids on ribosomes using the mRNA triplets as a template. Electroporation is the method of direct gene transfer by applying a pulse of high voltage applied to protoplasts/cells/ tissues to make transient pores in the plasma membrane which serve to uptake the foreign DNA. Transpiration is the loss of water from stomata. Thus, the correct answer is option E.
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