CameraIcon
CameraIcon
SearchIcon
MyQuestionIcon
MyQuestionIcon
1
You visited us 1 times! Enjoying our articles? Unlock Full Access!
Question

Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?

A
The eukaryotic DNA and plasmid DNA will have the same sequence
No worries! We‘ve got your back. Try BYJU‘S free classes today!
B
The eukaryotic DNA and plasmid DNA will have complementary sequences
Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses
C
The eukaryotic DNA can join with any plasmid DNA, regardless of sequence
No worries! We‘ve got your back. Try BYJU‘S free classes today!
D
The plasmid DNA can join with any eukaryotic DNA, regardless of sequence
No worries! We‘ve got your back. Try BYJU‘S free classes today!
E
The plasmid DNA and the eukaryotic DNA cannot join together due to differences in the structure of their DNA molecules
No worries! We‘ve got your back. Try BYJU‘S free classes today!
Open in App
Solution

The correct option is B The eukaryotic DNA and plasmid DNA will have complementary sequences

When a restriction enzyme like EcoRI cuts the two DNA strands it makes a staggered cut which results in the production of sticky ends. Many enzymes apart from EcoRI make a staggered cut that leaves a single-stranded tail at both ends. These tails on the DNA fragments generated by this restriction enzyme are complementary to the DNA cuts produced on the other fragment of DNA when digested by the same enzyme. These sticky or complementary ends help in transient base-pairing of the DNA fragments. Thus, the correct answer is option B.


flag
Suggest Corrections
thumbs-up
0
Join BYJU'S Learning Program
similar_icon
Related Videos
thumbnail
lock
Mitosis
BIOLOGY
Watch in App
Join BYJU'S Learning Program
CrossIcon