Question

How many antibodies are used in ELISA?

• A. 2
• B. 3
• C. 4
• D. 5

Answer 1

Answer: (A)

2

The diagnostic HIV test employs an ELISA to detect anti-HIV antibody in a human serum sample. Proteins from unknown HIV-infected cells are separated and in wells of ELISA plate. Then, serum to be tested is applied as a primary antibody, the free antibody has washed away and a secondary antibody linked to an enzyme substrate complex. The colour formation indicate the proteins to which the patient' serum contains antibody. Therefore, the correct answer is option A.