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Question

How many antibodies are used in ELISA?

A
2
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B
3
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C
4
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D
5
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Solution

The correct option is B 2
The diagnostic HIV test employs an ELISA to detect anti-HIV antibody in a human serum sample. Proteins from unknown HIV-infected cells are separated and in wells of ELISA plate. Then, serum to be tested is applied as a primary antibody, the free antibody has washed away and a secondary antibody linked to an enzyme substrate complex. The colour formation indicate the proteins to which the patient' serum contains antibody. Therefore, the correct answer is option A.

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