Question

Identify the correct sequence of steps in the construction of recombinant DNA.
$\left(1\right)$ use restriction enzymes
$\left(2\right)$ use DNA ligase
$\left(3\right)$ remove plasmid from parent bacterium
$\left(4\right)$ introduce plasmid into new host bacterium.

• A. $1-2-3-4$
• B. $4-3-2-1$
• C. $3-1-2-4$
• D. $2-3-1-4$

Answer 1

The correct option is D $2-3-1-4$

Generation of rDNA steps:

1) The DNA for insulin is first disengaged.

2) A plasmid made of DNA is expelled from a bacterial cell.

3) A limitation protein cuts the plasmid DNA open, leaving sticky finishes.

4) The insulin quality, with integral sticky closures, is included.

5) DNA ligase catalyst grafts (joins) together with the plasmid DNA and the Insulin DNA.

6) The plasmid (now hereditarily changed) is embedded again into the bacterium.

7) The bacterium has cell, partitions and delivers duplicates of the plasmid.

8) The Bacterium makes human insulin utilizing the quality in the plasmid.

9) The insulin is removed from the bacterial culture.

So, the correct option '2−3−1−4'.