In a bacterial transformation experiment, a scientist aimed to insert the human insulin gene into E. coli bacteria using a plasmid. Why must the restriction enzyme used in this experiment include recognition sites in the plasmid?
Restriction enzyme are specific to sites of recognition
Ligase will anneal the fragments of DNA created after using the EcoRI restriction enzyme.
Without the origin of replication, the plasmid will not replicate in bacteria and transformation efficiency will be low.
Including antibiotic resistant markers allow for selective screening to determine transformation efficiency.