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Question

In addition to auxins, ................ must be supplied to the culture medium to obtain a good callus in plant tissue culture.

A
ABA
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B
Cytokinins
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C
Gibberellins
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D
Ethylene
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Solution

The correct option is C Cytokinins
Callus is an unorganized, undifferentiated mass of diving cells formed during tissue culture which requires both auxins and cytokinins for its growth and differentiation. In callus, shoot regeneration is promoted by a cytokinin, such as BAP (Benzylaminopurine) and root regeneration is promoted by an auxin, such as NAA (Naphthalene acetic acid). Thus, shoot and root regenerations in callus are controlled by auxin-cytokinin balance. Usually, an excess of auxins promote root regeneration and that of cytokinins promote shoot regeneration.
So the correct answer is 'cytokinins'.

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