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Question

In reverse phase chromatography, the stationary phase is made :

A
nonpolar
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B
polar
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C
either nonpolar or polar
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D
none of these
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Solution

The correct option is A nonpolar

Reversed-Phase Liquid Chromatography ( RPLC) uses a hydrophobic or nonpolar stationary phase and a hydrophilic or polar mobile phase. The stationary phase is commonly composed of porous silica particles linked to alkyl chains (C4, C5, C8, C18) or other inert nonpolar substances such as divinylbenzene (DVB).


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