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Question

Match the following methods of cell disintegration with the tools they use.
i. Ultrasonication                             p. treating cells with organic solvents, alkalis and
detergents
ii. Enzymatic lysis                                  q. passing cell through narrow tube
iii. High pressure homogenization  r. lysozyme treatment
iv.
Chemical disruption                                        s. sound
  1. i-r, ii-q, iii-p,iv-s
  2. i-s, ii-r, iii-q, iv-p
  3. i- q,ii-r,iii-s,iv-p
  4. I-p,ii-q,iii-r,iv-s


Solution

The correct option is B i-s, ii-r, iii-q, iv-p
Cell disintegration is done using physical, chemical and enzymatic methods. Physical methods include ultrasonication and high pressure homogenization. Ultrasonication uses sound and high pressure homogenization uses high pressure where cells are passed through narrow tube with high pressure. In chemical methods, cells are treated with organic solvents, alkali or organic solvents. In enzymatic methods, enzymes like lysozyme are used to digest cell membranes and release intracellular product.

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