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Question

Meselson and Stahl's experiment is continued for three generations in bacteria. The ratio of 15N/15N:15N/14N:14N/14N containing DNA in the third generation would be

A
0 : 1 : 7
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B
1 : 4 : 0
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C
0 : 1 : 3
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D
0 : 1 : 4
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Solution

The correct option is C 0 : 1 : 3
Meselson and Stahl's experiment confirmed the semi-conservative mode of replication in DNA.

It means that when a DNA molecule undergoes replication, each strand of the parental DNA serves as a template and the new daughter strand is synthesized on the template strand such that each new molecule of DNA has one parental strand and one strand that is newly synthesised.


Keeping the semi-conservative replication in view, the ratio of 15N/15N:15N/14N:14N/14N comes out to be (0 : 1 : 3) as shown in the image below.
Hence, the correct answer is (0 : 1 : 3).

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