Name the positively charged & negatively charged components of the nucleosome.

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Solution

A nucleosome is composed of histone protein and DNA. DNA carries the negative charge due to the phosphodiester linkage and histone is basic in nature and carries a positive charge because histones are made up of basic amino acids. DNA wrapped around the histone octamer (H2A-H2B, H3, H4 two copies of each) through the H- bonding. So, the negatively charged component of the nucleosome is DNA and the positively charged components of nucleosomes are histone octamer.

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As DNA is negatively charged (or acidic) and histone are positively charged in their side chain(or basic).. Can we assume that nucleosome as a whole will be neutral neither acidic nor basic

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(iii) Histones are rich in the basic amino acid residues asparagine and valine
(iv) The packing of chromatin at higher level requires additional set of proteins that collectively are referred to as Non-histone chromosomal proteins.

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