Question

Pollen grains of plant whose 2n=28 are cultured to get callus by tissue culture method. What would be the number of chromosomes in the cells of the callus?

• A. 28
• B. 21
• C. 14
• D. 56

Answer 1

The correct option is C 14

Callus is an undifferentiated mass of cells obtained from the explant culturing by tissue culture method. The cells of the callus differentiate to form the entire plant. The chromosomes in the cells of the callus will be same as the number of chromosomes present in the explant. The diploid plant contains 2n = 28 chromosomes. When the pollen grains of this plant is used as the explant, the pollen grain contains the haploid (n) male gamete which is produced by meiotic division. So, the pollen grain will contain n = 14 chromosomes. This results in all the cells in the callus having 14 chromosomes.

Thus, the correct answer is option C.