Question

Polyethylene glycol method is used for

• A. Seedless fruit production
• B. Energy production from sewage
• C. Gene transfer without a vector
• D. Biodiesel production

Answer 1

Answer: (C)

Gene transfer without a vector

Polyethylene glycol (PEG) is a polyether compound. The compound has varied applications. Its biological uses include being used as a precipitant for plasmid DNA isolation and protein crystallisation. PEG is also used as a fusing agent in the transformation experiments. The gene or the desired fragment of DNA can be transferred into the host cell without the use of vector. PEG with calcium ions allows the membrane to become charged and allow the entry of the gene.

Thus, the correct answer is option C.