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Question

Regarding Assertion and Reason, select the right option
Assertion [A]: CaCl2 is necessary to create heat-shock competent cells.
Reason [R]: CaCl2 imparts a positive charge to the cell.
  1. [A] is true and  [R] is false.
  2. [A] is false and [R] is true .
  3. Both [A] and [R] are true and [R] is a correct explanation to [A].
  4. [Both [A] and [R] are true but [R] is not a correct explanation to [A].


Solution

The correct option is C Both [A] and [R] are true and [R] is a correct explanation to [A].
The cell membrane has a negative charge. This is not conducive for the transformation process. CaCl2 gives the cell an overall positive charge. This helps the cell to accept the negatively charged DNA.

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