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Question

Rupturing of the cell and digestion of the histone does not provide DNA. Why?

A
DNA stays dissolved in water
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B
Salt needs to be added for DNA to be extracted
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C
DNA cannot be clumped in aqueous solution
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D
DNA needs alcohol to be precipitated
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Solution

The correct options are
A DNA stays dissolved in water
B Salt needs to be added for DNA to be extracted
D DNA needs alcohol to be precipitated
DNA stays dissolved in aqueous solution after cell lysis and protease action. Salts are initially added to help precipitate DNA, following which ice-cold alcohol is added to the solution to accelerate the process. This technique is known as ‘salting out’.

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