Question

Select the option which correctly tells the sequence of production of Hepatitis B Vaccine?

i. Transformation of Yeast cell using vector

ii. Large scale production of HB antigens in the fermenter

iii. Production of vaccines for immunization

iv. Isolation of antigen producing gene

v. Purification of antigen

• A. iv → i → ii → v → iii
• B. iv → i → iii → ii → v
• C. iv → i → iii → v → ii
• D. iv → ii → i → v → iii

Answer 1

The correct option is A iv → i → ii → v → iii

Isolation of antigen producing gene - So that it can trigger the formation of antibodies against it.

Transformation of yeast cell using vector - A yeast cell is then transformed using vector carrying the isolated gene.

Large scale production of HB antigens in fermenter: The transformed yeast is grown in a fermentation tank which allows easier large scale production of the HB antigen.

Purification of antigen and its production: Antigens produced in large quantities are purified. Their production is on a large scale because these are used as vaccines commercially.