Question

Some of the steps involved in production of Humulin are given below. Choose the correct sequence.

(i) Synthesis of insulin gene artificially
(ii) Culturing recombinant E. coli in bioreactors
(iii) Purification of Humulin
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E. coli
(vi) Extraction of recombinant gene product from E. coli

• A. (i), (v), (vi), (ii), (iv), (iii)
• B. (i), (iv), (v), (ii), (vi), (iii)
• C. (iv), (iii), (v), (ii), (vi), (i)
• D. (i), (ii), (vi), (iii), (v), (iv)

Answer 1

The correct option is B (i), (iv), (v), (ii), (vi), (iii)

Some of the steps involved in production of Humulin are given below. The correct sequence are
(i) Synthesis of insulin gene artificially
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E. coli
(ii) Culturing recombinant E. coli in bioreactors
(vi) Extraction of recombinant gene product from E. coli
(iii) Purification of Humulin (human insulin)