Question

Southern blotting technique uses .......... paper for embedding DNA strands.

• A. Whatman's No. $1$
• B. Parchment
• C. Nitrocellulose
• D. Cellophane

Answer 1

The correct option is C Nitrocellulose

• Southern blotting is used to detect specific DNA sequences in a given sample of DNA.
• The first step in a Southern blot is to prepare the DNA mixture by breaking it into small fragments using a restriction enzyme.
• The mixture of DNA fragments is then separated according to size by gel electrophoresis.
• Then the double-stranded pieces of DNA are denatured into single strands within the gel.
• Next, the DNA is transferred from the gel onto a blotting membrane called the nitrocellulose membrane.
• The DNA is labeled using radioactive probes that can be detected by autoradiography.
  Thus the correct answer is option C.